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Plants from Test Tubes: An Introduction to Micropropagation (Fourth Edition)

By Lydiane Kyte, John Kleyn, Holly Scoggins, and Mark Bridgen

Thirty years ago, in vitro propagation was a new technique for producing plants, and Lydiane Kyte's Plants from Test Tubes became the standard work on the topic.

The new fourth edition has been thoroughly revised and updated to reflect the many advances in science and technology, including the five accepted sequential stages of micropropagation. Ten new plants have been added. This in turn has greatly expanded the already extensive bibliography. Among the new topics that have been introduced or expanded on are embryo culture for breeding, somaclonal variation, anther culture, somatic embryogenesis, cryopreservation, and genetic engineering. More ornamental plant examples are given and many new illustrations provided, including a chronology of discoveries in micropropagation.

About the authors

Lydiane Kyte

Lydiane Kyte (1919–2008) built a plant tissue culture program for a prominent woody ornamental lab and later developed a micropropagation business with her husband. Lydiane earned a B.S. in botany from the University of Washington.

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John Kleyn

John Kleyn (1923–2005) was an associate professor of biology at the University of Puget Sound in Tacoma, Washington, and author of a preeminent microbiology lab manual, now in its seventh edition. John received a Ph.D. in bacteriology from Cornell University.

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Holly Scoggins

Holly Scoggins is an associate professor of horticulture at Virginia Tech in Blacksburg and is director of the Hahn Horticulture Garden. She teaches courses in floriculture crops production, herbaceous landscape plants, greenhouse management, and public gardens maintenance and management. Holly holds a Ph.D. in horticultural science from North Carolina State University.

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Mark Bridgen

Mark Bridgen is a professor of horticulture at Cornell University and director of Long Island Horticultural Research and Extension Center. He earned a Ph.D. in plant physiology from Virginia Polytechnic Institute and State University.

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